spcas9 | Structural insights into a high fidelity variant of SpCas9

Keyword	CPC	PCC	Volume	Score	Length of keyword
spcas9	0.65	0.6	879	48	6

Keyword	CPC	PCC	Volume	Score
spcas9	1.13	0.9	8346	86
spcas9 structure	1.11	0.4	2358	81
spcas9 pam	0.63	0.2	7429	76
spcas9-ng	0.92	0.1	5895	82
spcas9 mrna	1.91	0.8	6172	42
spcas9-hf1	0.97	0.2	9492	15
spcas9 sgrna scaffold sequence	0.72	0.7	4256	14
spcas9 sacas9	1.74	0.7	4663	96

Search Results related to spcas9 on Search Engine

Structural insights into a high fidelity variant of SpCas9
nature.com

https://www.nature.com/articles/s41422-018-0131-6

WebJan 21, 2019 · Here, we present crystal structure of a SpCas9 variant, xCas9 3.7 that has broad PAM compatibility and high DNA targeting specificity, in complex with a single-guide RNA and its double-stranded...

DA: 42 PA: 8 MOZ Rank: 16

Continuous evolution of SpCas9 variants compatible with non-G …
nature.com

https://www.nature.com/articles/s41587-020-0412-8

WebFeb 10, 2020 · Nature Biotechnology (2024) The targeting scope of Streptococcus pyogenes Cas9 (SpCas9) and its engineered variants is largely restricted to protospacer-adjacent motif (PAM) sequences...

DA: 58 PA: 66 MOZ Rank: 47

Benchmarking of SpCas9 variants enables deeper base editor
nature.com

https://www.nature.com/articles/s41467-022-28884-7

WebMar 14, 2022 · Nature Communications - Numerous rationally-designed and directed-evolution variants of SpCas9 have been reported to expand the utility of CRISPR technology. Here the authors make comparisons of...

DA: 94 PA: 77 MOZ Rank: 55

Recent Advances in Improving Gene-Editing Specificity through …
nih.gov

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319960/

WebJul 13, 2022 · Streptococcus pyogenes Cas9 (SpCas9) is the most studied CRISPR–Cas9 nuclease for gene-editing applications, in part due to its short PAM requirement and high activity in eukaryotic cells. To address the issue that SpCas9 produces genome-wide off-target edits, researchers have developed a variety of high-fidelity …

DA: 85 PA: 38 MOZ Rank: 5

Structural and Dynamics Studies of the Spcas9 Variant Provide …
acs.org

https://pubs.acs.org/doi/10.1021/acscatal.2c01804

WebJul 6, 2022 · Here, we solved the apo form structure of a SpCas9 variant (xCas9 P411T) that harbors seven mutations in the SpCas9 REC region and one mutation in the C-terminal domain. This structure reveals that the mutations in the REC lobe resulted in a distinct conformation of the REC1 domain.

DA: 16 PA: 62 MOZ Rank: 45

Continuous evolution of SpCas9 variants compatible with non-G …
nih.gov

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7145744/

WebHere, we report the evolution of three new SpCas9 variants that collectively recognize NRNH PAMs (where R = A or G and H = A, C, or T) using phage-assisted non-continuous evolution (PANCE), three new phage-assisted continuous evolution (PACE) strategies for DNA binding, and a secondary selection for DNA cleavage.

DA: 13 PA: 67 MOZ Rank: 33

Engineered CRISPR-Cas9 nuclease with expanded targeting space
science.org

https://www.science.org/doi/10.1126/science.aas9129

WebAug 30, 2018 · Here, we report a rationally engineered SpCas9 variant (SpCas9-NG) that can recognize relaxed NG PAMs. The crystal structure revealed that the loss of the base-specific interaction with the third nucleobase is compensated by newly introduced non–base-specific interactions, thereby enabling the NG PAM recognition.

DA: 96 PA: 24 MOZ Rank: 8

The post-PAM interaction of RNA-guided spCas9 with DNA
science.org

https://www.science.org/doi/10.1126/sciadv.aaw9807

WebNov 13, 2019 · In the type II CRISPR-Cas system, the Cas9 protein from Streptococcus pyogenes (spCas9), which is in a complex with two RNAs (CRISPR RNA and trans-activating RNA), targets complementary 20–base pair (bp) sequences adjacent to a protospacer adjacent motif (PAM) in foreign DNA for site-specific double-stranded …

DA: 39 PA: 88 MOZ Rank: 46

A highly specific SpCas9 variant is identified by in vivo ... - PubMed
nih.gov

https://pubmed.ncbi.nlm.nih.gov/29431739/

WebAbstract. Despite the utility of CRISPR-Cas9 nucleases for genome editing, the potential for off-target activity limits their application, especially for therapeutic purposes. We developed a yeast-based assay to identify optimized Streptococcus pyogenes Cas9 (SpCas9) variants that enables simultaneous evaluation of on- and off-target activity.

DA: 61 PA: 24 MOZ Rank: 63

Engineering a PAM-flexible SpdCas9 variant as a universal gene ... - Nature
nature.com

https://www.nature.com/articles/s41467-021-27290-9

WebNov 25, 2021 · SpCas9 from Streptococcus pyogenes is the most extensively engineered Cas9 with robust and manifold functionalities. However, one inherent limitation of SpCas9 is its stringent 5′-NGG-3′ PAM ...

DA: 56 PA: 78 MOZ Rank: 31