Principle and Protocol of Sodium Dodecyl Sulphate …
Nov 17, 2015 · 7. Tris- glycine electrophoresis buffer: weigh 15.1g Tris and 94g glycine; Dissolve in 900ml deionized water; then add 50ml 10% (w/v) SDS and deionized water to 1000ml. Dilute 5-fold when using. The final concentration would be: Tris, 25mmol/L; glycine, 250mmol/L; SDS, 0.1% and the pH of the buffer is 8.3. 8.
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